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Author: Dr Tim Aung, Primary Care Practitioner, Brisbane & Logan, Queensland, Australia. DermNet NZ Editor in Chief: Adjunct A/Prof Amanda Oakley, Dermatologist, Hamilton, New Zealand. Copy edited by Gus Mitchell/Maria McGivern. February 2019.
Immunoglobulin E (IgE) is an antibody produced during a type I hypersensitivity reaction to an allergen. (See DermNet NZ's page Allergies explained.) A schematic for the type I reaction pathway is shown below .
IgE antibodies are normally found in small amounts in the blood. A higher level than normal infers an allergic disorder may be present .
Following exposure and re-exposure to an antigen (allergen) in susceptible individuals, a type I, or immediate, allergic reaction involves antigen-presenting cells, the activation of T-helper (Th) cells, the stimulation of B cells that release specific IgE, and the release of various pharmacological mediators (such as histamine, cytokines, leukotrienes, and others) from mast cells and basophils. The release of the mediators causes symptoms such as sneezing, wheezing, and weals.
Type 1 IgE-mediated hypersensitivity reaction pathway
APC, antigen-presenting cell; IgE, immunoglobulin E; Th, T-helper [cell].
Credit: Gaurab Karki, Microbiologist Kathmandu, via Online Biology Notes.
An IgE test is a blood test that detects circulating IgE. The test includes two types of test:
Total IgE and specific IgE tests can be ordered at the same time or independently.
IgE levels can be measured using one of several methods. The use of the radioallergosorbent test (RAST) to measure IgE has been superseded by the use of enzyme-linked immunosorbent assays (ELISA), fluorescent enzyme immunoassays (FEIA), and chemiluminescent immunoassays (CLIA) [3,4].
IgE blood tests
The colour of the top of the tube denotes what tests it is suitable for. In this case, the tube with the gold top (a serum separator tube) is used for IgE tests.
An IgE test is indicated when the taking of a careful patient history and an examination lead to a suspicion of type I allergy.
An IgE test can also be used for monitoring a patient with a known allergic condition, such as:
IgE tests are also useful to determine whether a patient is allergic to a specific protein, such as:
An IgE test can be used when skin prick testing is not available or is unsuitable; for example, in an individual with dermographism, extensive skin disease, recent use of antihistamines or systemic steroids, or when there is concern that prick testing could cause an anaphylactic reaction.
Although there is no absolute contraindication for IgE tests, the American Academy of Allergy, Asthma & Immunology, the Australasian Society of Clinical Immunology and Allergy, and Choosing Wisely make the following recommendations [5,6].
The specific allergens that can be tested can be classified as follows :
IgE tests are also available to test different mixes of allergens. These include a:
The total IgE reference range depends on the age of the individual (it ranges from 0 to 4 kU/L in a newborn and 0 to ~148 kU/L in an older child or adult).
The result of a specific IgE test is reported for a grouped allergen mix or an individual allergen. The table below shows how the results are typically rated and interpreted [2,8,9].
|Rating of specific IgE level (kUA/L)||Grade/Class||Interpretation|
|Absent or undetectable (< 0.35)||0||Unlikely|
|Low (0.35–0.69)||I||Doubtful significance|
|High (3.50–17.49)||III||More possible|
|Very high (17.50–49.99)||IV||More likely|
|Very high (50.00–100.00)||V||Very likely|
|Extremely high (> 100.00)||VI||Extremely likely|
This table shows arbitrary international reference figures. The actual reference range and grading vary with the laboratory and are based on the method used for the test, calibration, the age of the patient, and the type of allergens.
IgE test results should be carefully interpreted in the context of a patient’s presentation. High levels of total IgE can occur in allergic conditions, parasitic infections, certain immune-related disorders, and malignancies.
The sensitivity of specific IgE tests ranges from 60% to 95% and the specificity from 30% to 95%, depending upon the type of allergen and the age of the patient . There is a good predictive value (> 90%) for food (cow’s milk, egg, fish, and peanuts), pollens (grass and trees) and dust mites. Tests for some medicines, latex, moulds, and venom have poor sensitivity but greater specificity [3,4]. Note:
Further evaluation can be done by skin prick testing or by challenging the patient to a specific allergen in vivo.
Skin prick testing is more specific than IgE testing and gives a rapid result (often within 30 minutes), but it requires a trained practitioner and is not always tolerated by young children.
Specific IgE blood tests are simple and safe. They can be expensive, depending on the number of allergens tested. Caution is required when interpreting the results .
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