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Tzanck smear

Author: Dr Julie Fraser, Intern, Modbury Hospital, Adelaide, Australia; Chief Editor: Hon A/Prof Amanda Oakley, Dermatologist, Hamilton, New Zealand, October 2015.


What is a Tzanck smear?

The Tzanck smear test is a simple and cheap test that relies on viewing and interpretation of single cells (cytology).

Arnault Tzanck described the technique in 1947 to distinguish various blistering conditions.

The test is not often performed, due to the development of histology, virological culture, polymerase chain reaction (PCR) and electron microscopy.1

Indications for Tzanck smear

The Tzanck smear is mainly used in an acute setting to rapidly detect a herpes infection or to distinguish Stevens- Johnson syndrome / toxic epidermal necrolysis (SJS/TEN) from staphylococcal scalded skin syndrome. However, it can be used to diagnose a variety cutaneous infections and blistering diseases.

How is a Tzanck smear prepared?

Select a fresh blister on the patient.

  • Using a blunt scalpel blade, gently deroof the lesion
  • Scrape the base of the lesion
  • Smear the tissue onto a clean microscope slide
  • Allow it to dry in the air
  • Fix the specimen with preservative

Taking a Tzanck smear

What happens in the laboratory?

The slide is stained. The choice of stain depends on the sample and diagnostic possibilities.

Giemsa stain

Giemsa stain is poured over the slide. After 15 minutes, the slide is washed with sterile water.

  • Cell cytoplasm stains blue
  • Nuclei stain purple/red/pinkish

Toluidine blue

Toluidine blue is more rapid than Giemsa, as it only requires staining for 60 seconds.2

Tzanck smear interpretation

Characteristic cytological findings of Tzanck smears follow.3

Cutaneous infections

Tzanck smear of herpes simplex

Blistering diseases

Cutaneous neoplasms

The Tzanck smear test was found to have similar diagnostic accuracy to dermatoscopy in pigmented skin lesions.4

Inflammatory skin diseases

Advantages and disadvantages of Tzanck smear test

Initial results from a Tzanck smear should normally be confirmed by other techniques, such as biopsy or PCR.


Tzanck smear has the following benefits:

  • It is inexpensive
  • It results in minimal discomfort to patients
  • It is quick: useful for initial evaluation, or when rapid diagnosis required or in recurrences of disease. Faster diagnosis leads to early initiation of treatment.


Tzanck smear requires expertise:

  • In preparing the slide
  • In interpreting cytology

False negatives may occur in early or late disease.



  1. Weller R, Hunter H, Mann MW. Clinical Dermatology. [Internet] 5th Ed. Wiley, 11 January 2014 p39 [cited 20 September 2015]
  2. Kelly B, Shimoni T. Reintroducing the Tzanck smear. Am J Clin Dermatol. 2009;10(3):141–52. doi:10.2165/00128071-200910030-00001. PubMed
  3. Dey VK, Thawani M, Dubey N. Accuracy and reliability of Tzanck test compared to histopathology for diagnosis of basal cell carcinoma. Indian J Dermatopathol Diagn Dematol. 2015;2:8–13. Journal
  4. Durdu M, Baba M, Seçkin D. Dermatoscopy versus Tzanck smear test: a comparison of the value of two tests in the diagnosis of pigmented skin lesions. J Am Acad Dermatol. 2011;65(5):972–82. doi:10.1016/j.jaad.2010.08.019. PubMed
  5. Noyan MA, Durdu M, Eskiocak AH. TzanckNet: a convolutional neural network to identify cells in the cytology of erosive-vesiculobullous diseases. Sci Rep. 2020;10(1):18314. doi:10.1038/s41598-020-75546-z. PubMed

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