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A skin biopsy is the removal of a sample of skin. It is usually undertaken using a local anaesthetic injection into the skin to numb the area. The injection stings transiently. After the procedure, a suture or dressing may be applied to the site of the biopsy.
A skin biopsy may be deemed necessary as part of the diagnostic process. The additional information obtained from the biopsy can help identify diagnostic clues that are invisible to the naked eye.
The punch biopsy is generally the most useful type of biopsy. It is quick to perform, convenient, and only produces a small wound. It creates a full thickness sample of skin that allows the pathologist to get a good overview of the epidermis, dermis, and most of the time, the subcutis also.
A disposable skin biopsy punch is used, which has a round stainless steel blade ranging from 2–6 mm in diameter. The 3 and 4 mm punches are the most common sizes used. The clinician holds the instrument perpendicular to the anaesthetized skin and rotates it to pierce the skin. Using a forceps and scissors the skin sample is subsequently removed.
A suture may be used to close a punch biopsy wound or help control bleeding. If the wound is small, it may heal adequately without it.
A tangential shave of skin is taken using a scalpel, special shave-biopsy instrument or razor blade. No stitches are required. The wound forms a scab that should heal in 1–3 weeks.
As a shave biopsy does not include the full thickness of the skin, the drawback of such a biopsy is that it may be difficult for a pathologist to rule out or identify invasive disease.
A scoop biopsy is a deep form of shave biopsy, used to remove a skin lesion such as a benign mole by "scooping" it out. It is also called “saucerisation” or “tangential excision”. Because of the increased depth, this type of shave biopsy may lead to more extensive scarring if left to heal by secondary intention. In some cases, it may require stitches afterwards.
A skin curette may be used to scrape off a superficial skin lesion, such as a seborrhoeic keratosis. Some of the curettings are sent for histopathology. These samples are not suitable for determining if a lesion has been completely removed.
Incisional biopsies refer to removal of a larger and generally deeper ellipse of skin, using a scalpel blade. Stitches are usually required after an incisional biopsy. This type of biopsy may be useful to provide a better overview for the pathologist, which can improve diagnostic accuracy. It can also be useful when deeper layers or tissue are believed to be involved in the disease process (eg, subcutaneous fat or medium-sized blood vessels).
Excision biopsy refers to complete removal of a skin lesion, such as a skin cancer in which a margin of surrounding skin is taken to improve chances of complete removal. Smaller lesions are most often removed using a scalpel blade as an ellipse, with primary closure using sutures. Larger excisions may be repaired using a skin flap (moving adjacent skin to cover the wound) or graft (skin taken from another site to patch the wound).
It is crucial that the site of a biopsy is chosen carefully, or the pathological diagnosis could be incorrect or misleading. Here are a few guidelines to help find the best site, some general advice and pitfalls to avoid, depending on the type of skin lesion.
The clinician should ensure the pathology request form includes basic patient information (including age and identification details), the site and type of biopsy, and time and date. Left and right are best written out in full to avoid mistakes
In addition to this, it is crucial for the pathologist to be provided with clinical information and a range of possible diagnoses. For best clinicopathological correlation clinical information should include a description of the duration, symptoms and a dermatologic description.
The sample pot should be labelled with patient identification details, the body site of the biopsy, time and date, and checked against the request form for consistency. When multiple biopsies are taken roman numbers are best used to match the request forms with their corresponding sample pots.
Most skin biopsies are placed in formalin in a small pot and are sent to the lab for paraffin fixation, processing and histopathological examination.
Skin biopsy is usually straightforward and complications are uncommon. As a general rule, the larger the skin sample removed, the higher the chance of complications. The following complications might occur.
Intraoperative or postoperative bleeding can occur in anyone, but can be particularly troublesome in those with a bleeding tendency, or taking blood-thinning medications such as warfarin or aspirin.
Bacterial wound infection affects about 1–5% of excisional biopsies. It is however extremely uncommon in small punch, shave or incisional biopsies. Ulcerated or crusted skin lesions, site of biopsy, patient characteristics such as diabetes, older age, or use of immunosuppressive medicines may contribute to increased risk of infection.
The blade may cut a superficial sensory nerve causing pain or numbness. This is most likely to occur where the skin is thin, for example on the face or back of hand. Risk of motor nerve impairment is extremely rare, but can occur during skin cancer surgery in facial danger zones. These include the temporal, marginal mandibular, zygomatic branches of the facial nerve and the spinal accessory nerve (at Erb's point).
It is usual for a biopsy site to form a significant permanent scar. Some body sites such as the centre of the chest are prone to develop excessive or hypertrophic scars. This is also more common in Afro-Caribbean skin types.
Many biopsies are deliberately partial and only intended for diagnostic purposes. In excisional biopsies there is a risk of not removing the full lesion, which may recur later.
Allergies to local anaesthetics are a possibility but are also extremely rare. A vasovagal reaction is more common, which may cause the patient to faint and potentially hurt him or herself. Palpitations are another side effect which are related to the adrenalin commonly present in the local anaesthetic.
This is an uncommon complication of sutured wounds. It is more likely to occur in body sites where there is a lot of tension of the scar (eg, chest, back), immediately after suture removal, or as a result of infection. Avoidance of exercise, use of strappings and dissolvable sutures may help prevent this.
It usually takes about one or two weeks to obtain the result from the pathology laboratory, but can sometimes take longer if special stains or second opinions are required. The pathologist describes what is observed under light microscopy in several sections of the biopsy sample, and either makes a diagnosis or assists in differentiating between the suggested range of clinical diagnoses.
Skin diseases and conditions can at times be very difficult to diagnose accurately. In those cases the clinical and histopathological findings combined form a more complete picture to make a correct diagnosis. This is called the clinicopathological correlation. Many organisations hold regular multidisciplinary meetings (MDMs) at which clinical information, clinical photographs, and pathology slides are reviewed by a team of experts to determine the best diagnosis and treatment for the patient.
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