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Author: Dr Nick Love, PhD, medical student, Stanford University School of Medicine, Stanford, California, USA. Editor in Chief DermNet NZ: Adjunct A/Prof. Amanda Oakley, Hamilton, New Zealand. April 2019.
The information below is from an introductory course on dermatopathology intended for medical students and registrars/residents .
Haematoxylin (H) is a dark blue or violet histology stain that is positively charged, and binds to negatively charged basophilic substances, such as DNA and RNA. It can be considered a mostly nuclear stain. Conversely, eosin (E) is a negatively charged pink, red, or orange stain that highlights acidophilic (easily stained), positively charged substances, such as amino acid side chains (eg, lysine and arginine) that are relatively plentiful in the cytoplasm.
The H&E stain
The illustrations of normal skin in the figure below show comparatively thin skin from the wrist (bottom) and thicker skin from the bottom of the foot (left, an acral site). Note, only acral sites have a stratum lucidum (a clear layer of dead skin cells). Fine, spiny intercellular desmosomes in the stratum spinosum are also visible. Within the epidermis, non-keratinocytes have comparatively hyperchromatic nuclei.
Histopathology of normal skin
Seborrhoeic keratoses are well-demarcated proliferations of monotonous, sometimes cuboidal, epidermal keratinocytes. They are characterised by keratin-filled 'horn' cysts within the epidermis.
Histopathology of seborrhoeic keratosis
Psoriasis show acanthosis (epidermal hyperplasia), with flattened rete and parakeratosis (retention of keratinocyte nuclei in the stratum corneum). Neutrophils in the stratum corneum (Munro microabscesses) or upper epidermis (pustule of Kogoj) are pathognomonic. The epidermis is thinned above elongated dermal papillae.
Histopathology of psoriasis
Lichen planus classically exhibits a lichenoid inflammatory pattern (often described as a 'sawtooth' appearance) in which there are prominent mononuclear cells at the dermal–epidermal junction that cause damage. Basal keratinocytes may show vascular degeneration due to immune-mediated apoptosis. Other features are hypergranulosis (thickening of stratum granulosum), acanthosis, epidermal thinning, pigment incontinence (pigment within melanophages in the upper dermis), and spongiosis (oedema within the epidermis). (See Lichen planus pathology.)
Histopathology of lichen planus
There are several subtypes of basal cell carcinoma (BCC) in which there is a peripheral palisade of basal keratinocytes with hyperchromatic nuclei. In the figure below, the left lesion is a superficial BCC, and the right one is an infiltrative BCC. Following histological slide processing, retraction artefacts results in clefts. (See Basal cell carcinoma pathology.)
Histopathology of basal cell carcinoma
Dermatitis herpetiformis is a blistering disorder characterised by neutrophils at the tips of dermal papillae and subepidermal vesicles filled with neutrophils. Eosinophils, spongiosis, and perivascular inflammation are also typical. In this disorder, direct immunofluorescence reveals granular immunoglobulin (Ig)A in the papillary dermis. (See DermNet NZ's page on Dermatitis herpetiformis pathology.)
Histopathology of dermatitis herpetiformis
The pathological findings in drug eruptions are diverse and include many of the features seen in other types of lesions, including spongiosis, interface activity, and variable lichenoid inflammation.
Histopathology of drug eruption
Mycosis fungoides is a type of T-cell lymphoma characterised by epidermal T-lymphocytes with hyperchromatic nuclei that may form pathognomonic and Pautrier micro-abscesses. Other features seen in mycosis fungoides are perivascular lymphocytes, spongiosis, and surface serum crust. (See DermNet NZ's page on Mycosis fungoides pathology.)
Histopathology of mycosis fungoides
An actinic keratosis classically shows atypical basal keratinocytes and the 'flag sign' (alternating parakeratosis and orthokeratosis) that usually spares adnexal structures. Prominent epidermal hyperkeratosis, acanthosis, and solar elastosis are often present. The hypertrophic variant is notable for a thick stratum corneum.
Histopathology of actinic keratosis
Squamous cell carcinoma (SCC) in situ results in full-thickness atypia of keratinocytes. Typically there are parakeratosis, mitotic figures, acanthosis, and solar elastosis. Atypical squamous cells do not extend into the dermis. (See DermNet NZ's page on Squamous cell carcinoma in situ pathology.)
Histopathology of squamous cell carcinoma in situ
Cutaneous SCC results in dyskeratosis and invades beyond the basement membrane into the dermis. Cellular atypia may be described as glassy. Other features seen in cutaneous SCC include ulceration (serum crust) and keratin pearls. (See DermNet NZ's page on Squamous cell carcinoma pathology.)
Histopathology of squamous cell carcinoma
Melanocytic naevi can be intradermal, where there are proliferations (or 'nests') of melanocytes within the dermis, compound, where there are nests of melanocytes in the dermis and also junctional nests of melanocytes at the bottom of papillary rete; or junctional, where there are nests of melanocytes at the junction of the epidermis and the dermis, but no dermal melanocytes. A blue naevus is characterised by deeply pigmented spindle-cell melanocytes and lies underneath a relatively acellular dermal stroma.
Naevi with varying levels of atypical features may be called atypical or dysplastic due to architectural asymmetry, bridging of rete, wider junctional than dermal components, cellular atypia, fibroplasia, and lymphocytic infiltration. The images are from an acral site, showing a thick stratum corneum. (See DermNet NZ's pages on Melanocytic naevus pathology and Blue naevus pathology.)
Histopathology of melanocytic naevus
Melanoma is a malignant proliferation of atypical melanocytes. Histological features include a dusky cytoplasm, pagetoid scatter (abnormal cells within the higher levels of the epidermis), and irregular cellular nests. Melanoma in situ is confined to the epidermis whereas invasive melanoma extends past the basement membrane into the dermis. Reactive, perilesional lymphocytic inflammation is common. (See DermNet NZ's page on melanoma pathology.)
Histopathology of melanoma
The classical histology of molluscum contagiosum is epidermal hyperplasia, with a crater containing molluscum bodies (Henderson–Patterson bodies). These are large keratinocytes with eosinophilic intracytoplasmic inclusions that push aside the nucleus. (See DermNet NZ's page on molluscum contagiosum pathology.)
Histopathology of molluscum contagiosum
Two distinct subtypes of viral warts are shown in the figure below: verruca vulgaris (the common wart, left) and verruca plana (the flat wart, right). Verruca vulgaris is characterised by hyperkeratosis, hypergranulosis, parakeratosis, blood in the stratum corneum, and prominent acanthosis. The rete ridges point inwards. Large warts are highly vascularised with enlarged capillaries in the periphery and dermal papilla. Verruca plana are flat warts that lack hypertrophic features. Typically, there are koilocytes with a perinuclear halo in the upper epidermis. (See the DermNet NZ pages on Verruca vulgaris pathology and Verruca plana pathology.)
Histopathology of viral wart
Herpes simplex infection results in widespread keratinocyte necrosis and balloon cells with the '3 M’s' — multinucleation, moulding of nuclei (closely juxtaposed nuclei that conform to each other’s shape), and margination of chromatin. (See the DermNet NZ page on Herpes virus infection pathology.)
Histopathology of herpes simplex virus
Bullous pemphigoid is recognised by a subepidermal split, which generates tense bullae as seen in the figure below (the left image). The right-sided images in the figure show pre-cleft formation with a brisk eosinophilic infiltrate. Direct immunofluorescence shows the deposition of IgG or C3 along the basement membrane. (See DermNet NZ's page on Bullous pemphigoid pathology.)
Histopathology of bullous pemphigoid
Pemphigus vulgaris is a blistering disease in which there is acantholysis of keratinocytes above the basal layer of the epidermis (referred to as the 'tombstone' pattern). There is usually a superficial perivascular inflammatory infiltrate. Direct immunofluorescence shows the deposition of IgG between the epidermal keratinocytes creating a fishnet pattern. (See DermNet NZ's page on pemphigus vulgaris pathology.)
Histopathology of pemphigus vulgaris
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